| abstract |
An immunogenic cationic oil-in-water emulsion comprising emulsion particles containing an oil core that is in the liquid phase at 25 ° C and a cationic lipid, and a nucleic acid molecule that complexes with the emulsion particles, in which the cationic lipid is DOTAP, DOTMA, DOEPC, DSTAP, DODAC or DODAP, and the concentration of the cationic lipid in the oil-in-water emulsion is from 1.6 mg / ml to 25 mg / ml, in which the nucleic acid molecule it is a self-replicating RNA that encodes an antigen, and in which the average diameter of the emulsion particles is from 80 nm to 180 nm and the N / P of the emulsion is at least 4: 1, in which N / P is refers to the amount in moles of protonable nitrogen atoms in the cationic lipid divided by the amount in moles of phosphates in the RNA molecule; with the proviso that the nucleic acid molecule does not encode the secreted alkaline phosphatase, and the additional condition that the nucleic acid molecule is not an RNA encoded by plasmid A317, the sequence of which is shown in SEQ ID NO: 1. |