| abstract |
A method for the identification and detection of microorganisms in a sample, characterized in that it comprises: - carrying out at least one immunoassay reaction in a previously collected sample, to isolate and separate at least one selected target analyte which is a microorganism susceptible to be contained therein, incubating the sample with one or more reagents that are antibodies, each being specific for a target microorganism, conjugated with a magnetic particle, such that the target microorganism is coupled to the specific magnetic antibody during incubation and subsequently it is separated from the rest of the sample by means of magnetism; - carrying out a second immunoassay reaction in the sample previously subjected to the first immunoassay reaction, to label the microorganism previously coupled to the specific antibody conjugated with the magnetic particle before quantification thereof, incubating the sample with at least a second reagent, which is an antibody specific for the target microorganism and conjugated with a fluorescent label; and - detecting and quantifying the amount of the separated microorganism previously coupled to the antibodies by illuminating it and measuring the emitted fluorescence. |