http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2655509-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9d6f7da1e425c8a3ef1da21e08f4c196 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-501 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eac8528ff8127f64776c98aa38ac07cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5771bb875cb4725bc18cf64710d58392 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_163f974c4579f3039bbebbeb89444b87 |
| publicationDate | 2018-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2655509-T3 |
| titleOfInvention | Method for the relative quantification of the sequence, expression or copy changes of nucleic acids, using combined polymerase, ligation and nuclease reactions |
| abstract | A method of identifying the presence of one or more target nucleotide sequences in a sample, comprising: providing a sample potentially containing the one or more target nucleotide sequences; providing one or more sets of oligonucleotide probes, each set comprising (a) a first oligonucleotide probe having a specific 5 'portion of primer and a specific portion of the target, and (b) a second oligonucleotide probe having a 3 'primer specific portion, a specific portion of the target and a 5' nucleotide sequence, wherein said 5 'nucleotide sequence is complementary to at least a portion of the 3' specific primer portion and hybridizes with said portion complementary to the specific 3 'portion of primer to form a second bracketed oligonucleotide probe when said second probe does not hybridize with a target nucleotide sequence, wherein the first and second oligonucleotide probes of a set of probes are configured to be hybridize adjacent to each other in the target nucleotide sequence with a junction between the first and second oligonucleotide probes gives and, where, in a set of probes, the specific portion of the target of the second oligonucleotide probe has an identical overlap nucleotide at the junction with the first oligonucleotide probe; contacting the sample and the one or more sets of oligonucleotide probes under effective conditions so that the first and second oligonucleotide probes of a set of probes hybridize at adjacent positions in a specific base manner with their corresponding target nucleotide sequences , if present in the sample, where after hybridization the identical overlapping nucleotide of the second oligonucleotide probe forms a flap in the joint comprising the identical overlapping nucleotide; cleaving the identical overlapping nucleotide of the second oligonucleotide probe with an enzyme having 5 'nuclease activity, thereby releasing a phosphate at the 5' end of the second oligonucleotide probe; ligating the first and second oligonucleotide probes of the one or more sets of oligonucleotide probes together at the junction to form linked product sequences, wherein each linked product sequence comprises the specific 5 'primer portion, the specific portions of the target and the specific portions of 3 'primer of the first and second oligonucleotide probes of a set of oligonucleotide probes; detect the bound product sequences in the sample; and identifying the presence of one or more target nucleotide sequences in the sample based on said detection, optionally where the sample is selected from the group consisting of tissue, cells, serum, blood, plasma, amniotic fluid, sputum, urine, body fluids, body secretions, body excretions, circulating nucleic acids without cells, fetal nucleic acids without circulating cells in the pregnant woman, circulating tumor cells, tumor, tumor biopsy and exosomes. |
| priorityDate | 2012-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 228.