abstract |
A method of culturing mammalian cells that secrete heterologous proteins in a cell culture supernatant, in which the cell culture supernatant is maintained at a pH set at X + -0.05 in which X has a value of from 7.15 to 7.20, with the proviso that the pH is set to more than 7.10, in which the cell culture is a continuous culture maintained at a density of 1.4x106 to 2.8x106 cells / ml. |