abstract |
A method of culturing mammalian cells that secrete heterologous proteins in a cell culture supernatant, in which the cell culture supernatant is maintained at a temperature set at 36 ± 0.9 ° C, 5 in which the cell culture is a continuous culture maintained at a density of 1.4x106 to 2.8x106 cells / ml, and in which the mammalian cell is a CHO cell. |