| abstract |
A mouse whose endogenous immunoglobulin heavy chain locus is restricted such that it comprises a single human VH gene segment, one or more human DH gene segments, and one or more human JH gene segments and does not comprise a chain variable region locus heavy endogenous functional immunoglobulin; the mouse further comprises one or more human immunoglobulin VL gene segments operatively linked to one or more human JL gene segments, wherein the single human VH gene segment, one or more human DH gene segments, and one or more JH gene segments are linked operatively to a non-human immunoglobulin heavy chain constant region gene, wherein the single human VH gene segment is VH1-69 or a polymorphic variant thereof. |