| abstract |
The in vitro use of a compound having the following structure in the preparation of: a covalent conjugate between the compound and a bound carrier molecule that is a peptide, a nucleic acid, a protein, an antibody, a receptor, an enzyme, a drug, or is it a pesticide; or a covalent conjugate between the compound and a support that is controlled pore glass, glass plates, polystyrene, avidin coated polystyrene beads, acrylamide gel or activated dextran; the structure of the compound being a member selected from: ** (See formula) ** wherein X5 and X6 are members independently selected from H, C1-C6 substituted or unsubstituted alkyl, -OR ', - COOR', -NR ' R '', -SH, OP (OX3) N (X4) 2, wherein R 'and R "are members independently selected from the group consisting of H, and substituted alkyl or alkyl and wherein X3 and X4 are independently selected members of CN and C1-C6 substituted or unsubstituted alkyl; wherein at least one of X5 and X6 is a reactive group having the formula: wherein R9 and R10 are independently selected members of substituted and unsubstituted alkyl; X1 and X2 are members independently selected from -OH, -COOH, -NH2, -SH and -P (OX3) N (X4) 2; and wherein X3 and X4 are members independently selected from substituted and unsubstituted alkyl. |