| abstract |
A recombinant non-human organism genetically engineered to convert 5-aminopentanoate to a polyhydroxyalkanoate (PHA) polymer or copolymer thereof comprising 5-hydroxyvalerate, wherein the pathway for converting 5-aminopentanoate to PHA comprising 5 -hydroxyvalerate comprises 5-aminopentanoate transaminase (δ-aminovalerate transaminase), EC 2.6.1.48; succinate semialdehyde reductase (also known as glutarate semialdehyde reductase), EC 1.1.1.61; CoA-transferase, EC 2.8.3.14 and EC 2.8.3.n or Acyl-CoA synthetase, EC 6.2.1.3; and PHA synthase, EC 2.3.1.n; and wherein the recombinant organism produces the PHA comprising 5-hydroxyvalerate from lysine, wherein the recombinant organism is genetically engineered to express at least one or more genes encoding one or more enzymes for the production of the PHA comprising 5-hydroxyvalerate from lysine, wherein the enzymes are selected from the group consisting of: lysine 2-monooxygenase, EC 1.13.12.2; 5- aminopentanamidase (δ-aminovaleramidase), EC 3.5.1.30; 5-aminopentanoate transaminase (δ-aminovalerate transaminase), EC 2.6.1.48; succinate semialdehyde reductase (also known as glutarate semialdehyde reductase), EC 1.1.1.61; CoA-transferase, EC 2.8.3.14 and EC 2.8.3.n; Acyl-CoA synthetase, EC 6.2.1.3; PHA synthase, EC 2.3.1.n, in which 5-aminopentanoate is converted to 5-hydroxyvalerate, and in which 5-hydroxyvalerate is converted to a PHA polymer or copolymer thereof by the recombinant organism . |