http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2637825-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7a79a7677fc46be7e83ab1f3e065ed31 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-146 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8257 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2012-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a54d37ba396f67b5aee97b1c086b981b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da9bc8be421e6365af9083b4fa1ad233 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e7014c59b218b74e82d9fa947e002b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc08ea0c3b848d6f0000337289d3052d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_212aa22e1999e328320169c417c19704 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9eae9f459c28ce2055cab9c324e4749 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79fe68b15432cbeb325c5949a4e6cd00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09ac0c9ae92d39ad0cf723b65bcb9f48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ad60e2990b7fdc696b0cf5f595349b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_642f66bee0a257139dbeedd9cdc9b9a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82d2334cfc315a6e0318c52387a43364 |
| publicationDate | 2017-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2637825-T3 |
| titleOfInvention | Protein expression in plants |
| abstract | A method based on transient expression to produce large quantities of a heterologous polypeptide in Nicotiana tabacum comprising the steps of: (i) applying a necrosis test to preselect a variety, breeding line, or cultivating a Nicotiana tabacum plant and a strain selected from an Agrobacterium species, said variety, breeding line, or cultivating, shows less than 10% necrosis 5 days after the leaves of said variety, breeding line, or cultivating have been injected by syringe with the strain of selected Agrobacterium at a cell density of OD600 of 0.32, where necrosis is determined by measuring the areas of one or more necrotic leaves and the total areas of the one or more leaves that have infiltrated Agrobacterium cells ; (ii) infiltrate a complete plant of the variety, breeding line, or cultivate selected from Nicotiana tabacum with a suspension of the selected strain of the Agrobacterium species to an OD600 of between 0.1 and 4.0, where said strain comprises an expressible nucleotide sequence encoding the polypeptide; (iii) incubating the infiltrated plant for a period of between 5 days and 10 days under conditions that allow the expression of the expressible nucleotide sequence in the infiltrated plant and the accumulation of the heterologous polypeptide; and, optionally; (iv) further comprises after stage (iii), a stage (iv) to infiltrate the entire infiltrated plant with Agrobacterium with one or more enzymes that degrade the plant cell wall; wherein the heterologous polypeptide is expressed in an amount of at least 200 mg per kg of plant tissue mass |
| priorityDate | 2011-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 802.