| abstract |
Method for producing a hydrogel comprising hyaluronic acid, or salt thereof, crosslinked with divinyl sulfone (DVS), said method comprising the steps of: (a) providing an alkaline solution of hyaluronic acid, or salt thereof; (b) add DVS to the solution of step (a) with stirring, where the hyaluronic acid or salt thereof is crosslinked with DVS to form a gel; (c) treating the gel of step (b) with a buffer, where the gel swells and forms a hydrogel comprising hyaluronic acid or salt thereof, crosslinked with DVS, where the temperature of the solution in step (b) it is maintained in the range of 15 ° C - 30 ° C and then heated to 35 ° C - 60 ° C and the temperature is maintained in the range of 35 ° C - 60 ° C for a period of at least 5 minutes. |