abstract |
Mutant polypeptide comprising a mutated HBV polymerase domain with an internal elimination that functionally disrupts the activity of the polymerase, wherein said internal elimination is at most 30 amino acid residues, said mutated polymerase domain comprising the amino acid sequence represented in SEQ ID NO: 1, but lacking at least the Tyr residue at position 203, the Met residue at position 204, the Asp residue at position 205, the Asp residue at position 206, the Val residue at position 207, the Val residue at position 208 and the Leu residue at position 209, wherein said mutant polypeptide further comprises a mutated RNase H domain comprising a removal of at least 8 amino acids and at most 60 amino acids that include at least the part of SEQ ID NO: 3 that extends from the Glu (E) residue at position 39 to the Ala (A) residue at position 46. |