http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2626025-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1c7717103107509b5c106105e2056de2 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-465 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-905 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate | 2006-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a9d7def08aebe613ff0492e65eb33c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b6389a137e9b1ed1e3da82f5d6c4024 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3328606b68eedfef9fc236f9a074e854 |
| publicationDate | 2017-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2626025-T3 |
| titleOfInvention | Rationally designed meganucleases with sequence specificity and altered DNA binding affinity |
| abstract | A method for cleaving a double-stranded DNA target recognition sequence into a eukaryotic cell comprising: introducing into said eukaryotic cell a nucleic acid encoding a meganuclease; or a meganuclease protein; wherein said meganuclease cleaves said double stranded DNA target recognition sequence; and wherein said meganuclease is a recombinant meganuclease comprising: a polypeptide having at least 85% sequence similarity with residues 2-153 of the I-CreI meganuclease of SEQ ID NO: 1; and which has specificity for a recognition sequence semisite that differs by at least one base pair from a semisite in an I-CreI meganuclease recognition sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO : 3, SEQ ID NO: 4 and SEQ ID NO: 5, where the specificity at position -1: (i) has been altered to an A by a modification selected from the group consisting of D75C, D74L, D75Y, K139Y, T46A and T46C; (ii) to a G through a modification selected from the group consisting of T46E, T46D and T75E; or (iii) to a T by a modification selected from the group consisting of D75H, D75Q, D75Y, K139H, T46H and T46Q; (iv) to any base in a strand felt by a T46G modification. with the proviso that the method is not a method for the treatment of the human body or an animal by therapy; with the proviso that the method is not a method to modify the genetic identity of the germ line of human beings and with the additional caveat that the cell is not a human embryonic stem cell or a human blastocyst cell. |
| priorityDate | 2005-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.