http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2626014-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_27a71ddaaa2f5a8c228fc6cb3f3b3098 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1cc429c1084ae766e7451c8bbbf9de4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d560570bbb635bb185e11d032e133138 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_647e70fd9624764fe06c223197dfc68f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4862dd64ab805e44d6a062d5ff89a81b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ff5b19fe94b664501fe43c8d071febc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03287f6c2b677edd46d3405e86f5291c |
| publicationDate | 2017-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2626014-T3 |
| titleOfInvention | Oligonucleotide separation process of interest from a mixture |
| abstract | A method of separating a target oligonucleotide from a mixture of the target oligonucleotide and one or more impurities comprising: providing a two-phase liquid-liquid chromatography system mobile phase-stationary phase comprising a first liquid mobile phase and a liquid stationary phase containing at least one exchange substance that binds with the possibility of separation to the target oligonucleotide; causing the first liquid mobile phase to transport the target oligonucleotide in a flow in relation to and in contact with the liquid stationary phase in the column of a liquid-liquid chromatography apparatus so that the target oligonucleotide is attached to the exchange substance in the phase liquid stationary; then, moving the target oligonucleotide from the liquid stationary phase to a second liquid mobile phase that flows in relation to and in contact with the stationary phase through the column by means of a displacement substance capable of displacing the target oligonucleotide from the phase stationary liquid to the second mobile phase, characterized in that the exchange substance is a salt of a secondary, tertiary or quaternary organic amine with a counter anion, of general formula: R1R2R3R4N + X in which through the secondary, tertiary and quaternary sequence, respectively, two, three or four of the groups R1, R2, R3 and R4 are independently C1-20 alkyl or substituted alkyl such as fluoro- or trifluoromethyl alkyl or benzyl and the others are hydrogen, and X- is a halide anion. |
| priorityDate | 2011-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.