abstract |
A method of producing a composition for cartilage tissue repair, comprising the steps of (a) dissolving lyophilized fibrinogen in an aprotinin solution: (b) dissolving lyophilized thrombin in a stabilizing solution; (c) mixing a collagen solution enriched with thrombin and the stabilizing solution; and place the fibrinogen solution (a) on one side of a dual kit and the collagen-containing solution (c) on the other side and then mix (a) and (c), where the gel time after mixing (a) and (c) is 3 minutes and the maximum tension is greater than 10 N measured with a CR-500DX rheometer, where the fibrinogen concentration varies from 35 to 55 mg / L, the concentration of aprotinin solution is 1500 KIU / mL, the thrombin concentration is 29.41 IU / mL, the concentration of the stabilizing solution is 0.65 mg / mL, and the concentration of collagen is 13.23 mg / mL, where the stabilizing solution is Prepared by the addition of calcium chloride to the DMEM culture medium for cartilage cell culture and the DMEM culture medium comprises salts, amino acids and vitamins. |