http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2621390-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_131c120b2994d143a05e603805d04f10 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2522-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502723 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a880a0073378b41a24cd04f8fb87e7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_565cfd83f83a4756705759a4e7838d14 |
| publicationDate | 2017-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2621390-T3 |
| titleOfInvention | Oscillating amplification reaction for nucleic acids |
| abstract | A method of amplifying a nucleic acid target sequence template contained in a sample, comprising: contacting the sample with an amplification reaction mixture containing a primer complementary to the nucleic acid target sequence template and an agent nucleic acid destabilizer comprising at least one of DMSO, formamide and betaine, at a concentration of 8-15 percent by volume, the primer having a melting temperature> 55 ° C; oscillating a reaction temperature between a higher denaturation temperature and a lower hybridization temperature, in which a change in temperature is not greater than 20 ° C for a plurality of temperature cycles, the upper denaturation temperature being sufficiently high for completely denature the template; and amplify the template of the nucleic acid target sequence. |
| priorityDate | 2011-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 127.