http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2614811-T3

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filingDate 2010-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2017-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_22dd299dbc8165f12d177dd1e4e43d7c
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publicationDate 2017-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2614811-T3
titleOfInvention An assay to determine a molecular risk assessment of a complex polymicrobial sample suspected of containing an EHEC
abstract A process for carrying out a molecular risk assessment (MRA) on a sample suspected of containing an enterohemorrhagic Escherichia coli (EHEC), comprising the steps: a) contacting said sample or the DNA isolated therefrom with a pair of primers derived from at least the following target genes: - stx1; - stx2; and the following target genes: - espK or espK and eae; and with a pair of primers derived from at least one of the following target genes: - nleB; - nleH1-2; - nleE; - ent / espL2; and detecting the presence or absence of an amplification product for each of said target genes; and if the amplification products are detected for each of said genes of step a) then: b) contacting said sample or the DNA isolated therefrom with one or more pairs of primers derived from the eae target gene and determining the eae subtype.
priorityDate 2009-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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