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publicationDate 2017-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2609299-T3
titleOfInvention Solvent resistant diafiltration of peptides, PNA or oligonucleotides
abstract A process for the preparation of a first compound selected from oligonucleotides and peptide nucleic acids; the process comprising: (i) synthesizing the first compound by liquid phase synthesis in an organic solvent; and (ii) separating the first compound formed in step (i) from a second compound, which is a reaction byproduct of the synthesis of the first compound and / or an excess of a reagent used for the synthesis of a first compound, in where: the synthesis of the first compound in step (i) implies one or more coupling or deprotection reactions; the first and second compounds are dissolved in an organic solvent and separated by a diafiltration process using a membrane that is stable in the organic solvent and that provides a rejection for the first compound that is greater than the rejection for the second compound; The first compound is modified by subjecting it to one or more coupling and / or deprotection reactions after separation.
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