| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_39bf0bb8b07e151d4bcead749d7dd699 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-034 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-02036
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1077 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-34 |
| filingDate |
2010-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2017-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69d9fa0ee65f55d3ca22c3788a1238ea
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6f206c882267a2a4628a53edb099e69
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffbd36fee88bc3ade7d6b3d2fe6e713e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b8f2d997110b60a8097d1e41bee8ab8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b00d5a9b07ad6cfa88272c74210efa5 |
| publicationDate |
2017-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
ES-2609066-T3 |
| titleOfInvention |
Expression system |
| abstract |
A method of industrial production of a bacterial toxin expressed periplasmically by: A. in a discontinuous feeding phase, cultivating a culture of a gram-negative host cell comprising a polynucleotide comprising a part of 3 'toxin encoding a bacterial toxin and a 5 'signal portion encoding a polypeptide capable of directing the transport of said bacterial toxin to the periplasm of the host cell, in which the pH is 6.0-7.0; and B. inducing expression of said bacterial toxin at a pH that is and is maintained at a pH of 6.5-8.5, wherein said pH is at least 0.5 pH units higher than the pH in the stage A; and the substrate feed rate is maintained at between 20% and 80% of the substrate feed rate of stage A; in which the bacterial toxin has at least 90% sequence identity with SEQ ID NO: 32 and the procedure is carried out in a fermenter containing from 10-2000 liters of culture. |
| priorityDate |
2009-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |