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filingDate 2011-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2017-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2602500-T3
titleOfInvention Cell separation method
abstract A method for enriching and / or recovering the target cells from a sample containing the target cells, erythrocytes and other blood cells and / or thrombocytes, comprising: a) contacting the sample with a composition, said composition comprising: i) an erythrocyte aggregation reagent, wherein the erythrocyte aggregation reagent is selected from the group consisting of dextran, starch hydroxyethyl, methylcellulose or hydroxypropylmethylcellulose (HPMC) ii) at least one antigen binding fragment coupled to a particle magnetic, wherein said particle with said at least one antigen binding fragment specifically binds with at least one specific antigen for one or more of said other blood cells and / or thrombocytes; b) simultaneously apply i) gravity sedimentation for erythrocyte sedimentation; and ii) a magnetic field gradient to said sample for immobilization of said magnetic particle thereby generating a sediment and a supernatant phase, and c) recovering the target cells from the supernatant phase.
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