http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2601052-T3

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filingDate 2007-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2017-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2017-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2601052-T3
titleOfInvention Metabolic engineering of yeast cells that ferment arabinose
abstract A yeast cell capable of expressing the following nucleotide sequences, in which the expression of these nucleotide sequences confers on the yeast cell the ability to use L-arabinose and / or convert Larabinose into L-ribulose and / or xylulose -5-phosphate and / or in ethanol, in which yeast cell are expressed: (a) a nucleotide sequence encoding an arabinose isomerase (araA), wherein said nucleotide sequence is selected from the group consisting of: i . nucleotide sequences encoding an araA, said araA comprising an amino acid sequence having at least 80% sequence identity with the amino acid sequence of SEQ ID NO: 1. ii. nucleotide sequences comprising a nucleotide sequence that has at least 80% sequence identity with the nucleotide sequence of SEQ ID NO: 2; iii. nucleotide sequences whose complementary strand hybridizes with a nucleic acid molecule of sequence of (i) or (ii); (b) a nucleotide sequence encoding an L-ribulokinase (araB), wherein said nucleotide sequence is selected from the group consisting of: i. nucleotide sequences encoding an araB, said araB comprising an amino acid sequence having at least 80% sequence identity with the amino acid sequence of SEQ ID NO: 3. ii. nucleotide sequences comprising a nucleotide sequence that has at least 80% sequence identity with the nucleotide sequence of SEQ ID NO: 4. iii. nucleotide sequences whose complementary strand hybridizes with a nucleic acid molecule of sequence of (i) or (ii); and (c) a nucleotide sequence encoding an L-ribulose-5-P-4-epimerase (araD), wherein said nucleotide sequence is selected from the group consisting of: i. nucleotide sequences encoding an araD, said araD comprising an amino acid sequence having at least 80% sequence identity with the amino acid sequence of SEQ ID NO: 5. ii. nucleotide sequences comprising a nucleotide sequence that has at least 80% sequence identity with the nucleotide sequence of SEQ ID NO: 6; iii. nucleotide sequences whose complementary strand hybridizes with a nucleic acid molecule of sequence of (i) or (ii), in which in points iii. Hybridization is determined under hybridization conditions that allow a 200 nucleotide nucleic acid sequence to hybridize at a temperature of 65 ° C in a solution comprising approximately 1 M salt, and wash at 65 ° C in a solution of approximately 0.1 M, in which the hybridization is performed for 10 hours and the washing is done one hour with two changes of the washing solution.
priorityDate 2006-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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