| abstract |
An isolate of placental-derived cells obtainable from placental tissue after human delivery substantially free of blood, in which said cell self-renews and expands in culture, is multipotent, requires L-valine for growth, it grows in oxygen from about 5% to about 20%, and has the following additional characteristics: a) production of vimentin and alpha smooth muscle actin; b) expression of granulocyte-2 chemotactic protein (GCP-2) as detected by flow cytometry; c) lack of production of GRO-alpha and oxidized low density lipoprotein receptor, as detected by flow cytometry; d) production of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2 and HLA-A, B, C, as detected by flow cytometry; e) lack of production of CD31, CD45, CD80, CD86, CD117, CD141, CD 178, B7-H2, HLA-G, and HLA-DP, DQ, DR, as detected by flow cytometry; f) expression, which is relative to a human cell that is a fibroblast, a mesenchymal stem cell, or an iliac crest bone marrow cell, is reduced for short stature homeobox 2; 27kDa heat shock protein 2; chemokine (C-X-C with motifs) ligand 12 (stromal factor 1 derived from cells); elastin; DKFZp586M2022 cDNA (from clone DKFZp586M2022); mesenchymal homeobox 2; homologue of homeobox sine oculis 1; crystalline, alpha B; disintegrated associated activator of morphogenesis 2; DKFZP586B2420 protein; similar to neuralin 1; tetranectin; src homologous three (SH3) and the cysteine-rich domain; B 1 cell translocation gene, anti-proliferative; cholesterol 25-hydroxylase; transcription factor related to runt-3; hypothetical protein FLJ23191; interleukin 11 receptor, alpha; procollagen promoter of C-endopeptidase; curly counterpart 7; hypothetical gene BC008967; collagen, type VIII, alpha 1; tenascin C; Iroquois 5 protein homeobox; hephaestin; integrin, beta 8; synaptic vesicle glycoprotein 2; CDNA FLJ12280 fis, Clone of MAMMA1001744; Factor-1 receptor cytokine as; intermediate potassium / small calcium conductance activated channel, subfamily N, member 4; integrin, alpha 7; DKFZP586L151 protein; transcriptional co-activator with PDZ binding motif (TAZ); homologue of homeobox sine oculis 2; KIAA1034 protein; early growth response 3; non-distal homeobox 5; hypothetical protein FLJ20373; aldo-keto 1 reductase family, member of C3 (3-alpha hydroxysteroid dehydrogenase, type II); biglican; fibronectin 1; proencephalin; Type 1 beta integrin (with EGF same as repeat domains); cDNA clone EUROIMAGE 1968422; EphA3; KIAA0367 protein; natriuretic peptide receptor C / guanylate cyclase C (atrionatriuretic peptide receptor C); hypothetical protein FLJ14054; DKFZp564B222 cDNA (from DKFZp564B222 clone); membrane protein associated with vesicles 5; fibulin containing EGF as the extracellular matrix protein 1; BCL2 / adenovirus E1B 19kDa type 3 interaction protein; AE 1 binding protein; cytochrome c oxidase polypeptide subunit VIIa 1 (muscle); neuroblastoma, suppression of tumorigenicity 1; and insulin-like growth factor binding protein 2.36 kDa as tested by microassay; g) secretion of monocyte chemotactic protein 1 (MCP-1), interleukin-6 (IL-6), granulocyte chemotactic protein 2 (GCP-2), hepatocyte growth factor (HGF), growth factor of keratinocytes (KGF), epidermal growth factor-binding heparin (HB-EGF), matrix 1 metalloproteinase tissue inhibitor (TIMP1), thrombopoietin (TPO), Rantes (regulated in activation, normal T cells expressed and secreted) and thymus and activation-regulated chemokine (TARC), as detected by ELISA; h) lack of secretion of fibroblast growth factor (FGF), angiopoietin 2 (ANG2), platelet-derived growth factor (PDGF-BB), transforming growth factor beta 2 (TGFbeta2), macrophage inflammatory protein 1beta (MIP1b ), 1309, and macrophage-derived chemokine (MDC), as detected by ELISA; i) has the capacity to undergo at least 40 duplications of the growing population; and j) the expression, which in relation to a human cell that is a fibroblast, a mesenchymal stem cell, or an iliac crest bone marrow cell, is increased for a member of lectin superfamily of type C A2, Wilms tumor 1, aldehyde dehydrogenase member 1 of family A2, renin, oxidized low density lipoprotein receptor 1, protein zeta kinase, clone IM-AGE: 4179671, hypothetical protein DKFZp564F013, sub-regulated in ovarian cancer 1, and clone DKFZp547K1113 as tested by microassays. |