| abstract |
A chimeric polypeptide comprising a first part and a second part linked by a linker, wherein (a) said first part comprises an amino acid sequence of a bacteriocin cell binding domain (CBD); and (b) said second part comprises an amino acid sequence of at least one enzymatic active domain (EAD) selected from (i) the lytic domain of a bacteriophage endolysin, in which the lytic domain has at least 80%, preferably 90%, of amino acid sequence identity with the polypeptide of SEQ ID NO: 1; and (ii) the lytic domain of a bacterial autolysin, in which the lytic domain has at least 80%, preferably 90%, amino acid sequence identity with the polypeptide of SEQ ID NO: 3, in which the Polypeptide has lytic activity of bacterial cell wall. |