http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2590260-T3
Outgoing Links
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|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_84d9adaa7e7ce01ce8020f5c721aba10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41686a979f961bce6a9855b4b409a5b8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1472 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1497 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-0076 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-101666 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-0092 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5094 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-1475 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-49 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate | 1999-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_521c76e959b146d175e8bc84e9b34218 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97ba1aa41c177fa1dce8ccad630f346d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db2a470df64c6bf04f02f92d093d8a9d |
| publicationDate | 2016-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2590260-T3 |
| titleOfInvention | Determination of white blood cell differential |
| abstract | A method of identifying at least a subpopulation of target cells that contain nucleated material or that contain intracellular remnants of nucleated material, said target cells being contained in a quiescent sample of anticoagulated whole blood that is mixed with a fluorescent dye, and that is diluted in no more than about 1: 1; wherein said sample is dispersed is dispersed in a viewing chamber (14) that includes a region containing individually separated cells, said region having a variable thickness across the plane, as defined herein, ranging from approximately zero microns to about forty microns; wherein said dye is functional to differentially highlight at least three different types of white blood cells (34) in said sample; said method further comprising: a) the step of optical exploration of selected fields of vision (1, 3, 5) in said region, said fields containing individual red blood cells (32) and / or monolayers (31) of red blood cells and / or red blood cell aggregates (33); b) the stage of illumination of said selected fields of view (1, 3, 5) with an illumination source of one or more preselected wavelengths functional to cause said fluorescent dye to produce fluorescent emissions of nucleated material or remnant nucleated material of a plurality of known wavelengths, said emissions being characteristic of subpopulations of white blood cell types (34), wherein said preselected wavelengths include light in the range of 485 nm, and said known wavelengths include 530 nm, 585 nm, and 660 nm; and c) the step of analyzing said emissions at said plurality of known wavelengths to identify the subpopulations of cells in the fields of view; wherein the sample chamber (14) has opposing sample containment walls (4, 8), and the expression "through the plane" refers to a line of sight corresponding to the shortest distance between the walls in a given region of the chamber. |
| priorityDate | 1998-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.