http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2588223-T3

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filingDate 2009-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2016-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2016-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2588223-T3
titleOfInvention Procedure for the analysis of individual cells or particles in the blood using fluorescence and absorption of a dye
abstract A method for analyzing a blood sample comprising the steps of: depositing a blood sample in an analysis chamber (10) adapted to keep the blood sample at rest for analysis, the chamber (10) being defined by a first panel (12) and a second panel (16), both panels (12, 16) being transparent, the blood sample having one or more first constituents and one or more second constituents, the second constituents being different from the first constituents; mixing a dye with the blood sample, the dye being operative to make the first constituents and the second constituents emit fluorescence after exposure to a predetermined first light wavelengths, and the dye being operative to absorb light in one or more second predetermined wavelengths of light; illuminate at least a part of the blood sample containing the first constituents and the second constituents selectively with the first wavelengths and the second wavelengths; forming images of at least a part of the blood sample, including the production of image signals indicative of the fluorescent emissions of the first constituents and the second constituents and the optical density at the second wavelengths of the first constituents and of the second constituents; determine one or more fluorescent emission values for each of the first constituents and the second constituents using the image signals; determining one or more optical density values at the second wavelengths for each of the first constituents and the second constituents, the optical density being a function of the dye absorbed by the constituents, using the image signals; and identify the first constituents and the second constituents using the determined fluorescent emission and optical density values.
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