http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2587397-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eb596f03d029321340c965e75de6564a |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-575 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-605 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1077 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-10 |
| filingDate | 2010-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87f194a68a2db1aa4977e821a3a83518 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e7d1be5cdbc9102f57b9982ab2dfefd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd06aed2c065ddc1622a7d5e8e914cbc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c5a456c006de073f309bc9c92cf134b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7184110539ecf42dd3b5b9f9ca6a55fd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_998f6c50c3ab6954c7903cc6bf325e0e |
| publicationDate | 2016-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2587397-T3 |
| titleOfInvention | Procedure for preparing a site-specific conjugate of a physiologically active polypeptide |
| abstract | A process for preparing a site-specific physiologically active polypeptide conjugate, comprising the steps of: i) treating a physiologically active polypeptide with a non-peptidyl polymer in a reaction medium that contains a specific amount of an alcohol and has a specific pH to enable the non-peptidyl polymer to bind to a target site of the physiologically active polypeptide; and ii) isolating and purifying the physiologically active polypeptide conjugate from the reaction mixture of step i) by ion exchange chromatography using an alcohol, wherein the physiologically active polypeptide is an exendin, insulin, oxintomodulin, GLP- 1, GLP-2, ghrelin, calcitonin or one of the derivatives thereof having a structure selected from formulas (I) to (IV): ** Formula ** in which the alcohol is ethanol or isopropanol; wherein the alcohol is present in the reaction medium in an amount of 25% to 90% by volume, based on the total volume of the reaction medium; wherein the pH used in step i) is 7.0 to 10.0, when the physiologically active polypeptide is an exendin, oxintomodulin, GLP-1, GLP-2 or one of the derivatives thereof, and in The pH used in step i) is 4.0 to 10.0 when the physiologically active polypeptide is human insulin or a derivative thereof. |
| priorityDate | 2009-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 797.