| abstract |
Cells that can be obtained from human cord blood tissue substantially free of blood for use in inducing angiogenesis, wherein said cells are capable of self-renewal and differentiation, and have the following characteristics: a. potential to experience at least 40 duplications in the crop; b. binding and expansion in a coated or uncoated tissue culture vessel, wherein the coated tissue culture vessel comprises a coating of gelatin, laminin, collagen, polyiorithin, vitronectin or fibronectin; C. production of vimentin and actin of smooth muscle alpha; d. production of each of CD10, CD13, CD44, CD73, HLA-A, B, C, CD90, HLA-A, B, C, PD-L2 and, PDGFralfa, as detected by flow cytometry; e) increased expression of endogenous genes encoding interleukin 8; reticulon 1; chemokine ligand 1 (motif C-X-C) (melanoma growth stimulating activity, alpha); chemokine ligand 6 (motif C-X-C) (granulocyte chemotactic protein 2); chemokine ligand 3 (motif C-XC); and protein 3 induced by tumor necrosis factor alpha in relation to endogenous expression in a human cell that is a fibroblast, a mesenchymal cytoblast stem cell, or a bone marrow cell of the iliac crests, as characterized in a matrix gene; f) lack of production of CD31, CD34, CD45, CD80, CD86, CD 117, CD141, CD178, HLA-G, B7-H2 and HLADR, DP, DQ as detected by flow cytometry; g) growth of about 5% to about 20% oxygen h). require L-valine for growth; i) decreased expression of homeotic box 2 genes of short stature; 27 kDa heat shock protein 2; chemokine ligand 12 (motif C-X-C) (factor 1 derived from stromal cells); elastin; DKFZp586M2022 cDNA (from clone DKFZp586M2022); homeotic box 2 mesenchyme; homologue 1 of homeotic box of "sine oculis"; crystalline, alpha B; morphogenesis activator 2 associated with "disheveled"; DKFZP586B2420 protein; similar to neuralin 1; tetranectin; domain 3 homology with src (SH3) and rich in cysteines; B lymphocyte translocation gene 1, antiproliferative; cholesterol 25-hydroxylase; transcription factor 3 related to dwarfism; hypothetical protein FLJ23191; interleukin 11 receptor, alpha; procollagen C-endopeptidase enhancer; counterpart 7 of "frizzled"; hypothetical gene BC008967; collagen, type VIII, alpha 1; tenascin C; homeotic box protein iroquois 5; hephaestin; integrin, beta 8; synaptic vesicle glycoprotein 2; CDNA FLJ12280 fis, clone MAMMA1001744; factor 1 similar to the cytokine receptor; calcium-activated potassium channel of intermediate / low conductance, subfamily N, member 4; integrin, alpha 7; DKFZP586L151 protein; co-activator of transcription on the occasion of PDZ binding (TAZ); homologue 2 of homeotic box of "sine oculis"; KIAA1034 protein; early growth response 3; homeotic box of "distal less" 5; hypothetical protein FLJ20373; family 1 of aldo-keto reductases, member C3 (3-alpha hydroxysteroid dehydrogenase, type II); biglican; fibronectin 1; proencephalin; integrin, type beta 1 (with repeat domains similar to EGF); cDNA clone EUROIMAGE 1968422; EphA3; KIAA0367 protein; natriuretic peptide receptor C / guanylate cyclase C (atrial natriuretic peptide receptor C); hypothetical protein FLJ14054; DKFZp564B222 cDNA (from clone DKFZp564B222); membrane protein associated with vesicle 5; fibulin-like extracellular matrix protein 1 containing EGF; similar to protein 3 interaction with BCL2 / adenovirus E1B of 19 kDa; AE binding protein 1; polypeptide 1 of subunit VIIa of cytochrome c oxidase (muscle); neuroblastoma, suppression of tumorigenicity 1; and insulinoid growth factor binding protein 2, 36 kDa in relation to a human cell that is a fibroblast, a mesenchymal cytoblast, or a bone marrow cell of the iliac crests, as characterized in a gene matrix; j) express GCP-2 and NOGO-A as tested by immunocytochemistry; k) secrete MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO and TIMP1, as determined by ELISA; l. do not secrete SDF-1 alpha, TGF-beta2, ANG2, PDGFbb and VEGF, as determined by ELISA, wherein said angiogenesis induction comprises exposing a population of soft tissue cells to said cells that can be obtained from tissue of the umbilical cord. |