http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2582013-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f16e880838a5a337f595520a9936ca13 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-24 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
| filingDate | 2011-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b9b40762892052819f48ee18a9dd111 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f204f53f29452fdfb541ae2df083fe6a |
| publicationDate | 2016-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2582013-T3 |
| titleOfInvention | Rapid procedure for the detection of enzymes and microorganisms |
| abstract | Method of in vitro detection of an enzyme of a third generation cephalosporin resistant microorganism from a biological sample, said enzyme being selected for detection in the group consisting of extended spectrum b-lactamases, cephalosporinases and carbapenemases, said method comprising stages consisting of: a1) concentrating the microorganisms present in the biological sample, optionally after a step a0) of cultivation of the microorganisms; b1) suspending the microorganisms concentrated in step a1) in a solution comprising at least one chromogenic substrate capable of releasing a chromophore after hydrolysis by the enzyme for detection, said chromogenic substrate being the compound HMRZ-86 (acid trifluoroacetate (7R) -7- [2- (aminothiazol-4-yl) - (z) -2- (1-carboxy-1-methylotoxyimino) acetamido] -3- (2,4-dinitroestiryl) -3-cefem-4 -carboxylic, isomer E) or a salt thereof; c1) detect the possible release of the chromophore obtained in step b1); the chromophore release being detected in step c1) indicative of the presence of the enzyme for detection and further comprising said procedure, when the biological sample is a sample containing blood or red blood cells: - a stage of lysis of the red blood cells present in the biological sample before stage b1) of suspension or before stage a1) of concentration, and / or - a stage a ') of preparation of the biological sample before stage a1) of concentration of microorganisms, comprising the step a '): (i) the agglutination of the red blood cells, and (ii) the separation of the red blood cells from the microorganisms present in the sample. |
| priorityDate | 2010-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.