| abstract |
An isolated G-CSF polypeptide comprising a mutant peptide sequence, wherein the mutant peptide sequence encodes an O-linked glycosylation site that does not exist in a wild-type polypeptide corresponding to the isolated polypeptide, wherein the sequence of Mutant peptide is selected from the group consisting of MVTPLGP, MQTPLGP, MIATPLGP, MATPLGP, MPTQGAMPLGP, MVQTPLGP, MQSTPLGP, MGQTPLGP, MAPTSSSPLGP and MAPTPLGPA, or where the mutant peptide sequence is selected from the MTP group, PTPGP, MTP group MTPTQGP, MTPTSSP, M1TPQTP, M1TPTGP, M1TPLTP, M1TPNTGP, MTPLGP, M1TPVTP, M1TPMVTP and MT1P2TQGL3G4P5A6S7, or wherein the sequence of mutant peptide is selected from the group consisting P129ATQPT, P129TLGPT, P129TQGPT, P129TSSPT, P129TQGAPT, P129NTGPT, PALQPTQT, P129ALTPT , P129MVTPT, P129ASSTPT, MAP129ATQPTQGAM and MP129ATTQPTQGAM, or wherein the mutant peptide sequence is selected from the group consisting of LGIPTA P61LSSC, L GIPTQ P61LSSC, LGIPTQG P61LSSC, LGIPQT P61LSSC, LGIPTS P61LSSC, LGIPTS P61LSSC, LGIPTQP61LSSC, LGTPWAP61LSSC, LGTPFA P61LSSC and SLGAP58TAP61LSS, or wherein the sequence of mutant peptide is selected from the group consisting RHLAQTP175, RHLAGQTP175, QP175TQGAMP, RHLAQTP175AM, QP175TSSAP, QP175TSSAP , QP175TQGAMP, QP175TQGAM, QP175TQGA, QP175TVM, QP175NTGP and QP175QTLP, or wherein the sequence of mutant peptide is selected from the group consisting P133TQTAMP139, P133TQGTMP, P133TQGTNP, P133TQGTLP, PALQP133TQTAMPA, P129TTQP, P129NTLP, P129TLQP, P61TSSC, P61TSSAC, P61FTP and LGSTLGI. |