http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2555389-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8adaddc602ad7cb7f9e2d550681bb36d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate | 2010-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6444bfe1ee30955c20a923b98df9f79d |
| publicationDate | 2015-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2555389-T3 |
| titleOfInvention | Analysis of gene expression in individual cells |
| abstract | A method for preparing a cDNA library from a plurality of individual cells, the method comprising the steps of: (i) releasing mRNA from each individual cell to provide a plurality of individual mRNA samples, wherein the mRNA in each individual mRNA sample is from a single cell; (ii) synthesize a first strand of cDNA from the mRNA in each individual mRNA sample and incorporate a defined marker or combination of defined markers into each individual cDNA sample to provide a plurality of labeled cDNA samples, wherein each cDNA sample has a defined marker or combination of markers, wherein the cDNA in each labeled cDNA sample is complementary to mRNA from a single cell; (iii) pool the labeled cDNA samples; and (iv) amplifying the pooled cDNA samples to generate a cDNA library comprising double stranded cDNA. |
| priorityDate | 2009-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 106.