abstract |
A nucleic acid encoding an artificial primary miRNA transcript (pri-miRNA) consisting of, in order of position, a 5 'flanking region, a first siRNA region, a loop region, a second siRNA region, and a 3 'flanking region, in which the 5' flanking region comprises: (a) a 5 'binding sequence contiguously linked to the first siRNA region, wherein the 5' binding sequence consists of 5-7 nucleotides , (b) a bulge sequence 5 'positioned upstream of the binding sequence 5' consisting of 1-10 nucleotides, and (c) a spacer sequence 5 'positioned upstream of the bulge sequence 5', in the that the 5 'spacer sequence is UGGUACCGUU (SEQ ID No. 180). |