Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2d12aa5a14000a58c71db55cb965d22 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24222 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-14143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-5256 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-095 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-0011 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-04 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-095 |
filingDate |
2009-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2015-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adccf3f8bb9be337f1b82e0088404e37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb0077d5242c47c9a1208e33caa51318 |
publicationDate |
2015-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
ES-2546288-T3 |
titleOfInvention |
Method of purification of thermal shock protein complexes |
abstract |
A method for purification, from a mixture of origin, of at least one thermal shock protein complex formed by a thermal shock protein complexed with a peptide fragment, said method comprising the steps of: (i) providing a origin mixture comprising at least one complex of target thermal shock proteins; (ii) determining the isoelectric point (pI) of said at least one complex of target heat shock proteins to be purified from the mixture of origin; (iii) preparing a clarified cell lysate from the mixture of origin comprising said at least one complex of target thermal shock proteins; (iv) subjecting the cell lysate to purification using a solid ion exchange phase, in which the cell lysate is buffered using a buffer up to a pH at a distance of 2 units of the pI of said thermal shock protein complex target, and wherein a saline gradient is used to elute said at least one complex of target thermal shock proteins from the solid ion exchange phase; (v) identify an eluted fraction containing said at least one complex of target heat shock proteins based on pI. |
priorityDate |
2008-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |