http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2545760-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_81ad53b64eb74859acdc01278829b080 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-60 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5011 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57415 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2009-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64ede06129ca9bdb3658397df2cb225e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d87207ef7e4249c8993755c63ed911e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99288171296aef0574f4d354b224a373 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78eec8aedb2d2d62f95bd9cb63f0b4f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8a45b33b2bff10bb2f6cf6eb778fe78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f577167ad427d3f25efc4be67a815628 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a44551494f0ba35456c2423f95d495e |
| publicationDate | 2015-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2545760-T3 |
| titleOfInvention | Drug selection for breast cancer therapy using antibody matrices |
| abstract | Method comprising: (a) incubating isolated cells from a breast tumor with an in vitro anticancer drug, (b) lysing the incubated cells to produce a cell extract, (c) detecting an activation state of one or more analytes in the cell extract using an assay comprising a plurality of dilution series of specific capture antibodies of one or more analytes, in which the capture antibodies are immobilized on a solid support, and in which the assay comprises: (i ) incubating the cell extract with a plurality of dilution series of capture antibodies to form a plurality of captured analytes, (ii) incubating the plurality of analytes captured with detection antibodies comprising a plurality of antibodies independent of the activation state and a plurality of activation-dependent antibodies specific for the corresponding analytes in order to form a plurality of analytes detectable captured os, in which the independent activation state antibodies are labeled with glucosidase oxidase, in which the glucose oxidase and the independent activation state antibodies are conjugated with a sulfhydryl activated dextran molecule, in which the antibodies dependent on the activation state are marked with a first member of a signal amplification partner and in which glucose oxidase generates an oxidizing agent that is channeled and reacts with the first member of the signal amplification partner, (iii) incubate the plurality of captured analytes detectable with a second member of the signal amplification pair to generate an amplified signal, and (iv) detecting the amplified signal generated from the first and second members of the signal amplification pair, and (d ) determine whether the anticancer drug is suitable or not for the treatment of breast tumor by comparing the Activation status of one or more analytes with a reference activation profile generated in the absence of the anticancer drug, in which the method is intended to select an anticancer drug suitable for the treatment of a breast tumor, in which they are isolated cells from the mammary tumor of a subject who has not received treatment with the anticancer drug, and in which the reference activation profile provides the activation status of one or more analytes in breast tumor cells before treatment with the anticancer drug. |
| priorityDate | 2008-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 2030.