| abstract |
An in vitro method for controlling the properties of an Fc-containing molecule, which comprises altering the sialylation of oligosaccharides in the Fc region in the sialylated glycoform G2S2, in which the sialylation increases in the Fc region, in which the sialylation is Alters the non-mutant Fc region by enzymatic treatment or enzymatic modification of the molecule by an a- (2,3) -sialyltransferase, and in which the controlled properties are selected from the group consisting of affinity for one or more of the receptors FcgRI, FcgRIIA and FcgRIIIA, ADCC activity, macrophage or monocyte activation, serum half-life and greediness. |