abstract |
Hybrid polypeptide comprising a first amino acid sequence comprising at least one catalytic module with alpha-amylase activity and a second amino acid sequence comprising at least one carbohydrate binding module, wherein said first amino acid sequence is at least 75% , at least 80%, at least 85%, at least 90% or even at least 95% identity with the alpha amylase catalytic domain of Rhizomucor pusillus in SEQ ID NO: 20, and wherein said second amino acid sequence is a CBM that has at least 75% identity with a CBM selected from the group consisting of Aspergillus niger glucoamylase CBM in SEQ ID NO: 96, Aspergillus kawachii glucoamylase CBM in SEQ ID NO: 94, or A. glucoamylase CBM rolfsii in SEQ ID NO: 92. |