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filingDate 2012-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2015-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2542408-T3
titleOfInvention Method of determining the amount of at least some fatty acids contained in different biological materials of the same animal raised for meat production
abstract Method for determining the amount of at least some fatty acids contained in different biological materials of the same animal raised for meat production, from a database in which they have been previously determined, for a reference material from of the same type of animal, the fatty acid compositions or profiles, determined by gas phase chromatography, and then the corresponding infrared absorption or Raman diffusion spectra have been measured and recorded, the method comprising the following steps: a / a calibration equation corresponding to the determination, by a spectroscopic method, of a fatty acid or of a sum of fatty acids is determined by a mathematical model, for a sample of this reference material; b / the use of said calibration equation of the previous stage is validated, with a view to its use with new samples to be analyzed, from the moment in which for a series of samples called "validation", of the same nature as the biological reference material, the correlation coefficient r2 between the content of the fatty acid considered or the sum of fatty acids obtained by gas phase chromatography and by spectroscopy, is at least equal to 0.7; c / a new sample to be analyzed, of the same nature as the reference material, is subjected to light radiation to obtain its absorption spectrum, and the calibration equation validated above is used to deduce its profile, that is, the acid content so-called "main" fats, namely those with a correlation coefficient r2 at least equal to 0.7; characterized in that it also comprises at least one of the following steps: d / the profile is determined, that is to say the content of fatty acids called "secondary", that is to say all those whose coefficient r2 is less than 0.7, from the "main" fatty acid profile as determined in the previous stage, by means of statistical prediction equations that have a correlation coefficient r2 at least greater than the correlation coefficient r2 determined between the values of secondary denominated fatty acids ", measured by spectroscopy and the values obtained by gas phase chromatography, or by means of statistical prediction equations that have a correlation coefficient of at least equal to 0.7, obtained with the gas phase chromatography method; e / the fatty acid content of at least one other material of the same animal is determined, from the profiles obtained in stages c / and / od /, by means of statistical prediction equations that have a correlation coefficient for less equal to 0.7, obtained with the gas phase chromatography method.
priorityDate 2011-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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