| abstract |
A high performance liquid chromatography method comprising: loading a solution containing metal complexes in a column, in which the metal complexes are selected from mimetic compounds of superoxide dismutase, MRI image enhancement agents, catalase mimetics and catalyst catalysts. peroxynitrite decomposition, eluting the metal complexes of the column with a mobile phase, said mobile phase comprising a salt of a coordination anion in a solvent system, the amount of the coordination anion salt being in excess sufficient for the anion of coordination saturate all the binding sites of interchangeable ligands in the metal of the metal complexes, directing the formation of a single species during elution with the mobile phase, and detecting the metal complexes with a detector, in which the salt comprises the minus a selected salt of sodium chloride, lithium chloride, chloru Potassium ro, ammonium chloride, tetrabutylammonium chloride, sodium thiocyanate, potassium thiocyanate, ammonium thiocyanate, lithium thiocyanate, potassium acetate, sodium acetate, ammonium trifluoroacetate, lithium acetate, potassium formate, formate sodium, ammonium formate, lithium formate, sodium cyanate, potassium cyanate, ammonium cyanate, potassium carboxylate, sodium carboxylate, lithium stearate, sodium stearate, sodium azide, potassium azide or lithium azide. |