| abstract |
A recombinant Gram negative bacterial cell comprising a mutant spr gene, which encodes a UniprotKB / SwissProt P0AFV4 spr protein, which has a mutation in one or more amino acids selected from N31Y, R62C, 170T, Q73R, C94A, S95F, V98E, Q99P, R100G, L108S, Y115F, D133A, V135D, V135G, L136P, G140C, R144C, H145A, G147C and H157A, and where the cell has reduced activity of the Tsp protein, specific tail protease, compared to a natural type cell . |