| abstract |
Isolated variant polypeptide with glycoside hydrolase activity, which has an identity degree with amino acids 1 to 513 of SEQ ID NO: 2 of at least 90%, preferably at least 95%, and most preferably at least 97%, where the amino acid sequence of the polypeptide differs from SEQ ID NO: 2 in at least one position corresponding to position 94 of amino acids 1 to 513 of SEQ ID NO: 2, where the difference in less one position is a substitution at position 94 and the variant has improved properties compared to the progenitor glycoside hydrolase, where the improved properties are selected from the group consisting of temperature-dependent activity profile, thermostability, pH activity, stability of pH and substrate specificity, product specificity and chemical stability. |