| abstract |
Method for producing a polypeptide, comprising: (a) culture of a mutant of a parent strain of Fusarium venenatum in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and pyrG, amyA and alpA, where pyrG, amyA and alpA are modified causing the deficiency of the mutant strain in the production of orotidine-5'-monophosphate decarboxylase, alpha amylase and alkaline protease, respectively, compared to the parent strain of Fusarium venenatum when grown under identical conditions ; and (b) recovery of the polypeptide from the culture medium. |