http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2538123-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_57f3e2af72b2933c004ed61dfccaaee1 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-583 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2009-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_458b788163675b839ba806a8d756ed91 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fc9ee94e58629db06821b805ea6a78d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56d0fbac9f5486c46204d871ef90e3e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c134f883e3f9694b67f59469632ced62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df4089308afc4224ba367dcdf02e8581 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b2ef5d3ce26a68067255100213ce239 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d67b4ca4ffff98b55f949cc22e8907f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92297fbc0c9dc33288526840fc986588 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc744e9b3eaa40f5d6da85348d7dbd69 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d57e69f46cee711605f48aa1cd92219a |
| publicationDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2538123-T3 |
| titleOfInvention | Method for chromogenic detection of two or more target molecules in a single sample |
| abstract | A method for the chromogenic detection of two target molecules in a single tissue sample consisting of: contacting the tissue sample with a first specific binding moiety that specifically binds to a first target molecule; detect the first target molecule in the tissue sample by depositing an aromatic, insoluble, electron-rich chromogenic product, where the chromogenic deposition consists of reacting 3,3'-Diaminobenzidine (DAB) with a catalyst to directly or indirectly form the product aromatic chromogen, insoluble, rich in electrons; contacting the tissue sample with a second hapten-labeled specific binding moiety that specifically binds to a second target molecule, wherein the hapten of the second hapten-labeled specific binding moiety is dinitrophenol; Treating the tissue sample with a solution containing a soluble, electron-rich aromatic compound containing naphthol phosphate is performed before or at the time the second dinitrophenol-labeled specific binding fraction is contacted with the tissue sample. detecting the second target molecule by depositing a second insoluble chromogenic product that can be distinguished from the electron-rich, insoluble aromatic compound deposited to detect the first target molecule, wherein treating the tissue sample with the solution containing the aromatic compound soluble, electron-rich, reduces background staining resulting from non-specific binding of the dinitrophenol-labeled specific binding moiety to the insoluble electron-rich compound deposited near the first target molecule. |
| priorityDate | 2008-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 526.