http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2534955-B1

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filingDate 2013-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2016-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59e70a0a89afc8bc50fb13e6baea3d80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be852636099a35d69e110b1bb193fe5b
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publicationDate 2016-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2534955-B1
titleOfInvention Environmental toxicity bioassay based on phycobionts isolated from lichen
abstract Environmental toxicity bioassay based on phycobionts isolated from lichens. # The present invention contemplates a method for assessing the toxicity of a sample based on a) obtaining a population of phycobionts in a sterile culture medium suitable for each type of assay. ; b) the preparation of a suspension with the population of phycobionts obtained in a) in a suitable culture medium; c) the exposure of the suspension obtained in b) to the sterilized sample whose toxicity is to be evaluated, d) the incubation of the suspension of phycobionts isolated from lichens (c) under conditions of temperature between 5 and 25 ° C, and a photoperiod of 8 at 12 h of light and 8 to 12 h of darkness of white light intensity between 5-200 {mi} mol {sup, m-2} s {sup, -1} for a suitable period (between 0 and 100 hours) , according to sample a); e) measurement of the optical dispersion of the culture obtained in step d); f) quantification of the DNA of the sample obtained in d); g) determination of the autofluorescence of the chlorophyll of the sample obtained in step d).
priorityDate 2013-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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