http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2532746-R1

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filingDate 2013-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5244d9e6f586266bdce1a366c3ef7f4
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publicationDate 2015-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2532746-R1
titleOfInvention Computer-implemented method for dynamic characterization of cells in cell cultures and computer programs to carry out the method
abstract Method implemented by computer for dynamic characterization of cells in cell cultures and computer programs to carry out the method. # The method comprises acquiring a sequence of fluorescence images of a cell culture; segment said fluorescence image sequence and identify the location, shape and size of each cell that is part of the culture; measure the fluorescence level of each identified cell to determine a concentration of a calcium ion in each identified cell; stimulate said culture at an external stimulation frequency through an external supply of electrical energy; determine the response of each identified cell to said stimulation frequency; and determine a global response of said stimulated culture by means of said external input of electrical energy and analyze the electrical propagation of said stimulus in the form of a local elevation of the concentration of the calcium ion at said stimulation frequency.
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Total number of triples: 23.