| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eb7ce8745175865c6357f7d5fe7fccf5 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-3809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-361 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-282
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-3847
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-165
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-048
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-3804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-06 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-36 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-38
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-282
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-22 |
| filingDate |
2004-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2015-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e5fa12426d4db1ec5df9bf410ff1d86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9373c9e5377106352dcd9f49ee3ed219
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fcfca2b5db253037bc67d6f4fc54bc97 |
| publicationDate |
2015-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
ES-2530446-T3 |
| titleOfInvention |
Removal of high molecular weight aggregates using hydroxyapatite chromatography |
| abstract |
A method of purifying at least one antibody monomer of an antibody preparation containing high molecular weight aggregates comprising subjecting the antibody preparation to hydroxyapatite chromatography, in which hydroxyapatite chromatography comprises: contacting a resin of hydroxyapatite in a column with the antibody preparation, in which the column is equilibrated with a equilibration buffer having a pH of 6.4 to 7.4 and comprising 1 to 20 mM sodium phosphate and 0 to NaCl 200 mM, and, - optionally washing the column with a wash buffer having a pH of 6.4 to 7.4 and comprising 1 to 20 mM sodium phosphate and 0 to 200 mM NaCl, and, - eluting the purified antibody of the resin with at least one elution buffer having a pH of 6.4 to 7.6 and comprising 1 to 20 mM sodium phosphate and 0.2 to 2.5 M NaCl. |
| priorityDate |
2003-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |