http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2528892-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6a9b318b4860e3a53f6e30085c3d4373 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-622 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1055 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1082 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1241 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2012-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dad85dd0d577e241d1b2bcfc363ceaab |
| publicationDate | 2015-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2528892-T3 |
| titleOfInvention | Identification mediated by transposition of specific binding or functional proteins |
| abstract | A method for identifying a polypeptide having a desired binding specificity or functionality, comprising: (i) generating a diverse collection of polynucleotides, preferably plasmid vectors or PCR amplicons, of double stranded DNA, encoding polypeptides having different binding specificities or functionalities, wherein said polynucleotides comprise a sequence encoding a polypeptide arranged between a first and a second sequence of inverted terminal repeats that are recognized by at least one transposase enzyme and are functional therewith; (ii) introduce the diverse collection of polynucleotides of (i) into host cells; (iii) expressing in the host cells at least one functional transposase enzyme with said inverted terminal repeat sequences, such that said diverse collection of polynucleotides is integrated into the genome of the host cell to provide a population of host cells expressing said collection diverse polynucleotides encoding polypeptides that exhibit different binding specificities and functionalities; (iv) exploring said host cells to identify a host cell that expresses a polypeptide with a desired binding specificity or functionality; and (v) isolating the polynucleotide sequence encoding the polypeptide of said host cell. |
| priorityDate | 2012-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 98.