| abstract |
An improved heterogeneous immunoassay for the detection or quantification of an analyte using a hydrophilic acridinium compound, with high quantum yield, containing electron donating functional groups at the C-2 position and / or the C-7 position of the acridinium ring, where the high quantum yield acridinium compound has a relative quantum light yield greater than 1 when compared to a corresponding acridinium compound without electron donating functional groups at the C-2 and / or C-7 position of the acridinium ring, in which case the electron donating functional group is selected from the group consisting of -OCH2CH2CH2SO3 - and -O (CH2CH2O) n-CH2-CH2-OMe, where n> = 0-5. |