| abstract |
A method to detect two or more different targets in a sample, consisting of the following: contacting the sample with two or more specific binding fractions that specifically bind to two or more different targets, where the two or more fractions of specific binding are conjugated with different haptens, at least one of the haptens being a first rotenone isoxazoline with the formula where R-R5 are, independently, hydrogen, aldehyde, alkoxy, aliphatic, substituted aliphatic, heteroaliphatic, amino, amino acid, amido, cyano (-CN), halogen, hydroxy, hydroxylamine, oxime, oxime ether, hydroxyl alkyl, ketone, nitro, sulfhydryl, sulfonyl, sulfoxide, carboxy, carboxylate, ester, alkyl ester, acyl, exomethylene, ether, cyclic, heterocyclic , aryl, alkyl aryl, heteroaryl, carbohydrate, monosaccharides, disaccharides, oligosaccharides, polysaccharides and combinations thereof; And it is oxygen, an amine or sulfur; and at least one of the R-R5 substituents is attached to a linker or to a carrier molecule, the other hapten being an oxazole, a pyrazole, a thiazole, a benzofurazan, a triterpene, a urea, a thiourea, a nitroaryl other than dinitrophenyl , a second rotenone isoxazoline other than the first rotenone isoxazoline, a coumarin, a cycloolignan, a heterobaryl, an azoaryl or a benzodiazepine; contacting the sample with two or more different anti-hapten antibodies that can be detected separately; and detect anti-hapten antibodies. |