http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2434542-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_80042b861c194eec0ac987bd2eab39a5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
| filingDate | 2009-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e010a68909e0fb94d2659fda4e8d128 |
| publicationDate | 2013-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2434542-T3 |
| titleOfInvention | Amplification and sequencing of targets with primers comprising triplex forming monomer units |
| abstract | A procedure comprising the steps of: a. Provide a template nucleic acid b. Provide a first oligonucleotide c. Provide a polymerase d. Provide nucleotide triphosphates e. Mix the components of stages ad and provide conditions that allow the primer to hybridize with the template - in which the first oligonucleotide is an oligonucleotide between 5 and 60 nt in length, comprising a TINA monomer of the formula Z, in which Z is described by the general structure: X-L-I1-C-I2 -in which X is a structural monomer unit that can be incorporated into the structure of an oligonucleotide or a nucleotide analogue, or PNA, or analogues of PNA, L it is a linker, I1 is a first intercalator comprising at least an essentially flat conjugate system, which is capable of co-stacking with nucleotide bases of DNA, RNA or analogs thereof, C is an optional conjugate and I2 is a second intercalator that it comprises an essentially flat conjugate system, which is capable of co-stacking with nucleotide bases of DNA, RNA or analogs thereof f. Under conditions that allow primer extension, prolong the first oligonucleotide hybridized with the template. |
| priorityDate | 2008-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 146.