http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2434491-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d0e31ad71844db57564151f365e120d3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-4742 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2474-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-25125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-962 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-743 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6875 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5748 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 |
| filingDate | 2006-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d11105045b3097164cfd0faf267cbba8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d69b5a808b22c3a85290945d80848467 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82ef3af761bcac7b81f77c6f757e4ba2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7bad6c5955e93c708c5fd8dacb2b73d |
| publicationDate | 2013-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2434491-T3 |
| titleOfInvention | In situ multiplex immunohistochemical analysis |
| abstract | An immunohistochemical method to simultaneously detect the presence or absence of at least three targets in a biological sample, comprising the steps of: (a) performing antigen recovery in the biological sample, wherein said antigen recovery comprises the steps: i. dewax and rehydrate the biological sample; and ii. bring to a boil in antigen recovery buffer; (b) perform autofluorescence reduction in the biological sample before stage (a), stage (c) or stage (d), wherein said autofluorescence reduction comprises incubating said biological sample in a solution comprising hydrochloric acid 1 % and 70% ethanol; (c) contacting the biological sample with (1) a first specific antibody for a first target, (2) a first labeling reagent wherein said first labeling reagent comprises an antibody binding moiety for said first antibody and a first detection moiety, (3) a second specific antibody for a second target, (4) a second labeling reagent comprising an antibody binding moiety for said second antibody and a second detection moiety, (5) a third specific antibody for a third target, and (6) a third labeling reagent comprising an antibody binding moiety to dichotercer antibody and a third detection moiety; and (d) detecting each of said first, second and third labeling reagents, respectively, in biological sample, whereby the presence of said first, second and third labeling reagents, respectively, indicates the presence of said first, second or second third targets, respectively, in biological sample and the absence of said first, second or third labeling reagents indicates the absence of said first, second or third targets, respectively, in said biological sample, wherein said biological sample is human tissue included in paraffin. |
| priorityDate | 2005-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 561.