http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2433718-T3
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5bc5292a07e34d16eb1d033a16d3aec3 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2009-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d57fd1d87fcf7e11293f8a8e153f2a34 |
publicationDate | 2013-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | ES-2433718-T3 |
titleOfInvention | Quantitative Monochromatic Multiplex PCR |
abstract | A method for determining the copy number of a first target nucleic acid and a second target nucleic acid, comprising: a) contacting a first target nucleic acid with a first set of primers and a second target nucleic acid with a second set of primers and adding a single detection marker to form a reaction mixture in a homogeneous system, in which the number of copies of the first target nucleic acid sequence is greater than the number of copies of the second target nucleic acid sequence , b) amplify by the polymerase chain reaction the first target nucleic acid with the first set of primers to form a first amplicon having a first melting temperature (Tm) and amplify by the second polymerase chain reaction target nucleic acid with the second set of primers to form a second amplicon having a second Tm, wherein said second Tm is greater than di cha first Tm; c) during the polymerase chain reaction, determine the amount of the detection marker at a first acquisition temperature, at which said first signal acquisition temperature is below said first Tm; d) increasing the temperature of the reaction mixture to a second signal acquisition temperature and determining the amount of the detection marker, wherein said second signal acquisition temperature is above said first Tm and below said second Tm; e) repeat steps (b) to (d) at least once; and f) determining the relative copy number of said first and said second target nucleic acids. |
priorityDate | 2008-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 682.