Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3434865446cb768b1dc8dcb327ffb857 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-052 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-16643 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-4727 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-0393 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
filingDate |
2012-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2014-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a00abeb0ed5db70615f759c900bcc1c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b76dd03f84e523c54c93fb2497af9bf3 |
publicationDate |
2014-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
ES-2427854-B1 |
titleOfInvention |
Calcium sensors and methods for the detection of intracellular free calcium |
abstract |
Calcium sensors and methods for the detection of intracellular free calcium. # The invention provides a calcium sensor based on a fusion protein comprising (i) a first polypeptide domain capable of binding Ca {sup, 2 +}, (ii ) a second polypeptide domain of the sequence SEQ ID NO: 1 (GFPuv) or a variant thereof that maintains two maximums in its excitation spectrum and at least one maximum in its emission spectrum, and (iii) a flexible peptide that unites the first domain and the second domain covalently. It also provides a transgenic non-human animal that comprises the fusion protein. Also, the invention provides methods for detecting the intracellular Ca {sup, 2 +} calcium concentration. |
priorityDate |
2012-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |